Configuration of Fortessa Laser Detector Band Pass Filter Dichoric Filter Color name available on the instrument Additional Color Channel in LSRII Blue 488nm (BV570 filter change) Yes F 450/50 Blank Pac Blue/V450/BV421 DAPI/Sytox Blue/Fixable Violet Yes Red 633nm

provide easier access to bandpass filters and mirrors, simplifying changes to experimental setup. To improve experimental efficiency, the optional BD™ High Throughput Sampler (HTS) allows sample loading from 96- or 384-well microtiter plates for screening assays. Boundless Potential The BD LSRFortessa Analyzer 4 5 BD High Throughput Sampler (HTS)

Table of lasers and filters available: Laser Filters BD LSRFortessaTM Filters Features The standard configuration of the BD LSRFortessa™ can be changed in order to detect fluorophores with emission peaks not covered by the standard filter set. In the following list you can see all additionally available filters with some fluorophores, covered better by the alternative filter… LSR Fortessa . The LSR Fortessa offers 5 lasers and is able to detect up to 18 parameters (fluorochromes) at the same time! Technical Highlights Lasers 355 nm, 20 mW laser power 405 nm solid state diode, 50 mW laser power 488 nm solid state, 50 mW laser power 561 nm, 640 nm , 40 mW laser power Flow cell 180 x 430µm rectangular quartz flow cell Configuration of Fortessa Laser Detector Band Pass Filter Dichoric Filter Color name available on the instrument Additional Color Channel in LSRII Blue 488nm (BV570 filter change) Yes F 450/50 Blank Pac Blue/V450/BV421 DAPI/Sytox Blue/Fixable Violet Yes Red 633nm If you change filter(s) on particular channel(s) for your experiment, please make sure to switch back to default settings and put the spare filter(s) back to the storage place; When you are done with the experiment be sure to wash BD LSRII (or BD LSR-Fortessa) properly: “Run” at … LSR Fortessa - software parameters and fluorochromes Laser Collection Filter (bandpass) Diva Parameter Name Fluorochromes 488nm Blue 530/30 530/30 Blue FITC, Alexa 488, eGFP, CFSE, Dye Cycle Green 670/30 30 Blue PerCp 710/50 710/50 Blue PerCP-Cy5.5, PerCp-eFluor 710, PerCp provide easier access to bandpass filters and mirrors, simplifying changes to experimental setup. To improve experimental efficiency, the optional BD™ High Throughput Sampler (HTS) allows sample loading from 96- or 384-well microtiter plates for screening assays. Boundless Potential The BD LSRFortessa Analyzer 4 5 BD High Throughput Sampler (HTS) BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a.

4. Remove the sheath container lid. 5. LSR Fortessa - software parameters and fluorochromes Laser Collection Filter (bandpass) Diva Parameter Name Fluorochromes 488nm Blue 530/30 530/30 Blue FITC, Alexa 488, eGFP, CFSE, Dye Cycle Green 670/30 30 Blue PerCp 710/50 710/50 Blue PerCP-Cy5.5, PerCp-eFluor 710, PerCp 633nm Red 670/14 670/10 red 2016-11-05 · Laser Name Dichroic Filter Bandpass Filter Flourochromes FSC None None FSC PerCP-Cy5.5 690 LP 695/40 PerCP, PerCP-Cy5.5, PE-Cy5.5, PerCP-eFluor 710, PE-Alexa700, PI, 7AAD FITC 505 LP 530/30 FITC, Alexa 488, GFP, YFP, CFSE, LIVE/DEAD Fixable Green, DyeCycle Green SSC None 488/10 SSC QD 605 595 LP 610/20 QD 605, BV605 Am Cyan 495 LP 525/50 BD LSR Fortessa (16 colour Analyser) 4 lasers, 16 filters (see table below).

BD LSR Fortessa Standard Operation Protocol Refill Sheath Container 1. Make sure the flow cytometer is in STANDBY mode. 2. Disconnect the air line (green) and fluid line (Blue) from the sheath container. 3. Depressurize the sheath container by pulling up on the vent valve. 4. Remove the sheath container lid. 5.

BD LSRFortessa. It houses 5 lasers and has a similar configuration as our BD Fortessa. fluorescence detectors with optical filters optimized for the detection of FITC, PE, PerCP, BD LSRFortessa™ X-20.

Lasers and filters configuration available in our BD Fortessa II for Class II work.

Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Site Terms and ** Additional filter changes can be requested. Contact us for more information BD- LSR Fortessa Configuration Violet (405nm) Blue (488nm) Yellow-Green (561nm) Red … 488nm Laser (Blue) DetectorFilterMirrorDye A 710/50 685 LP PerCP, PerCP-Cy5.5 B 530/30 505 LP FITC, GFP, CFSE, Alexa Fluor 488 C 488/10 - SSC 561nm Laser (Yellow/Green) DetectorFilterMirrorDye A 780/60 750 LP PE-Cy7 B 710/50 685 LP PE-Cy5.5 C 670/30 635 LP PE-Cy5 D 610/20 600 LP PE-Texas Red, PE-CF594 E 585/15 - PE 640nm Laser (Red) DetectorFilterMirrorDye A 780/60 750 LP APC-Cy7, APC-efluor 2016-11-05 BD LSR Fortessa (16 colour Analyser) Contact us. BD LSR Fortessa (16 colour Analyser) 4 lasers, 16 filters (see table below). Sample loading via tubes.

BD LSRFortessa™ and.
Hur många bor i bangladesh

I am still likely to replace my aging LSR II with a second Fortessa X20. Review date: 08 Dec 2015 | BD LSRFortessa X-20 The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa Fortessa X20c - Fluorescence Detection Map Fluorochromes (common examples) Detector Name Filter Range Laser Wavelength Ultraviolet (355 nm) BUV395 UV379 379/28 BUV496 UV525 525/50 Violet (405 nm) BV421, Pacific Blue, DAPI V450 450/50 Pacific Orange, BV510 V525 525/50 BV605 V610 610/20 BV650 V670 670/30 BV711 V710 710/50 BV785 V780 780/60 Blue > > > > We have a 2014 BD Fortessa SORP, and have recently seen dramatic and > persistent stability problems following use of the PRIME function. We > think we have found a solution to this problem, and are wondering if anyone > else has seen it. 2013-06-27 · With the industry's most advanced high throughput plate loader and hotel, the S1000 family of analyzers go head-to-head with BD FACS Canto, BD LSR II, and BD Fortessa at a fraction of the cost and size while offering a superior solution for conventional and high throughput flow cytometry applications.

Flödande cell Bd fortessa x20 I testläge.
Gatukontoret stockholms stad

hur gammal ska man vara for att kora epa
halsvenstas symtom
billig semester danmark
gammal hund som flåsar
lönsam kundutveckling
andrew lundgren

The BD LSRFortessa optical layout allows for up to four lasers. Laser power Photomultiplier tube (PMT) with a 488/10 bandpass filter. Emission optical design .

Line. Filter.

Vardeutveckling villor
hl bil

The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry simultaneously with a defined set of optical filters that meet or exceed the

Cat. No. Blue. 488 nm. 530/30.

BD LSR Fortessa (16 colour Analyser) 4 lasers, 16 filters (see table below). Sample loading via tubes. Table of lasers and filters available: Laser Filters

Laser. Detector name.

Group User and Password 5) If working with bacteria, open the black nob in front of the FSC. 6) Open the optical filter compartment (on the left side of the Fortessa and two small ports in front) to make sure that the filters necessary for detecting your fluorophores are in place. BD Fortessa Laser Detector name (band pass filter) *Suggested Fluorochromes Blue (488nm) FSC SSC 488 (530/30) Fitc, Alexa488, GFP, YFP 488 (695/40) PerCPCy5.5 BD FACSArray™ bioanalyzer Green Diode 532 Yellow PE Far Red PerCP-Cy5.5 PE-Cy7 Red Diode 635 Red APC Alexa Fluor® 647 Infrared BD APC-H7 APC-Cy7 *BD FACSCalibur™ flow cytometry system Argon 488 FL1 Green FITC Alexa Fluor® 488 FL2 Yellow PE FL3 Red PE-Cy5a PerCP PerCP-Cy5.5 PE-Cy7 Red Diode 635 FL4 Red APCa Alexa Fluor® 647 *BD FACSCanto™ 1.844.8.BD.LIFE (844.823.5433) Now with one customer support number, it's easier for you to review your order status, place a new order, get product information or learn about product availability. BD support quick links filter Side Scatter Detector Photomultiplier tube (PMT) with a 488/10 BP filter Emission Optical Design Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays.